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b51d-04 and sc=biogeosciences

HR: 11:05h
AN: B51D-04
TI: The interplay of pH and CO$_{2}$ on intracellular metal content in {\it Thalassiosira weissflogii}
AU: * Wei, L
EM: wei@imcs.rutgers.edu
AF: IMCS, Rutgers University, 71 Dudley Rd, New Brunswick, NJ 08901 United States
AU: Sherrell, R M
EM: sherrell@imcs.rutgers.edu
AF: IMCS, Rutgers University, 71 Dudley Rd, New Brunswick, NJ 08901 United States
AB: How does the metal composition of phytoplankton relate to the metal composition of their ambient environment? Are there other aspects of water chemistry or growth conditions that substantially influence the overall metal composition of living cells? How does the magnitude of the resulting variability compare to differences in the metal contents among species and major phytoplankton taxonomic groups? To address these questions, we will present a study of how pH and CO$_{2}$ (aq) interplay on the uptake of bioactive trace metals Fe, Mn, Cu, Zn, Co, Ni, Zn, Cd and Mo, adding to the more well documented influences of absolute and relative metal availability. First we compare intracellular metal content in {\it Thalassiosira weissflogii} (Tw) grown in modified Aquil media with high and low Zn and Cd and bubbled with different CO$_{2}$-air mixtures. Then we examine the temporal variations in intracellular metal content of Tw during metal buffered batch culture without pH and pCO$_{2}$ control. In total, we argue that the metal composition of a diatom is dependent on multiple interacting aspects of the growth conditions. The links and disconnects between Fe, Mn and Cu content and photosynthetic efficiency, and between Zn, Cd and Co content and carbon acquisition enzymes will be discussed.
DE: 4805 Biogeochemical cycles (1615)
DE: 4825 Geochemistry
DE: 4835 Inorganic marine chemistry
DE: 4855 Plankton
DE: 4875 Trace elements
SC: Biogeosciences [B]
MN: 2004 AGU Fall Meeting