%********************************************************************************** % % CalC version 5.4.0, script "FCT_Fig4.par" % Victor Matveev, January 4, 2005 % % "Facilitation through Buffer Saturation: % Constraints on Endogenous Buffering Properties" % V. Matveev, A. Sherman and R. Zucker % Biophys. J. (2004) 86:2691-2709 % % This script reproduces Figs. 2 and 4 of the Manuscript; it accepts a % command-line parameter spesifying the total concentration of the buffer. For % instance, run "calc FCT_Fig4.par 500" (or "calc FCT_Fig4.par 500 | xmgr -pipe") % to reproduce Figs. 2 and 4B. % %********************************************************************************** path = "" % If running under Windows, specify here the path to the % directory containing the script imported below file = path "FCT_main.par" include file % Import the simulation parameters from the main script, % which defines everything but the buffer's properties: Buffer.total = $2 % Concentration is given by the 2nd command-line parameter % (the 1st parameter, '$1', is the script filename itself) Buffer.D = 0.2 % Diffusion coefficient is 0.2 um^2/ms Buffer.KD = 0.5 % Affinity = 0.5 uM Buffer.kplus = 0.8 % Binding rate = 0.8 / (uM ms) plot Ca2 % Plot [Ca2+] and [Buffer] concentrations at location "2" plot Buffer2 % Uncomment one of the following, depending on your output preferences: % plot.print "FCT.Btotal" $2 "uM" % Filename prefix will indicate the Btotal value plot.method xmgr % plot.method gl