Week 5

Chromatographic Theory

Important Topics

Stationary and mobile phases
partition coefficient and capacity factor (K and k')
Column efficiency and what affects it
Resolution
Programming
Quantitation and calibration methods

Important Skills

Understand and explain the basis of chromatographic separations
Know how changes in phases, column size or packing, etc. will affect the separation
Understand the principles governing column programming (either temperature or mobile phase) and when and why it is needed
Be able to carry out calculations to calibrate a chromatographic system and quantitate analytes

 

Textbook chapter on Chromatography

Some overheads

Homework solutions

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